Preventive role of fresh garlic on high-fat diet induced fatty liver in albino rats

Obesity has become a worldwide problem due to westernization of diet, even in developing countries leading to consumption of high-fat diet. One of the most common diseases resulting from use of high-fat diet is fatty liver. Garlic is one of the oldest herbs used for medicinal purposes since ancient times. So the present study was undertaken to observe the protective role of fresh garlic on high-fat diet induced fatty liver in albino rats and correlate the results with previous studies. Thirty adult albino rats, weighing from 200-240 gram were taken for this study. The rats were divided into 3 groups according to dietary regimen, Group A received control diet, Group B received high- fat diet [20 ml corn oil in 100 gm of diet] and Group C received high-unsaturated fat diet with fresh garlic [20 ml corn oil with 6 gm fresh garlic in 100 gm of diet], for 8 weeks at the end of which they were sacrificed and tissue slides stained with haematoxylin and eosin. There was marked increase in weights in rats receiving high fat diet. Haematoxylin and eosin stained sections showed moderate fatty infiltration with empty spaces in hepatocytes with absent or pyknotic nuclei in high-fat group which were preserved to a great extent in group receiving fresh garlic along with high-fat diet. This study has proved that use of fresh garlic along with high-fat diet prevents its damaging effects in liver to a great extent

Effect of piroxicam and zinc on morphological and histochemical changes in mice liver

To observe the morphological and histochemical findings, produced by piroxicam and zinc in mice liver and correlate with serum hepatic enzyme. For this experimental study 30 adult mice [25 -30 grams] were obtained from animal house of Jinnah Postgraduate Medical Center, and divided into three groups i.e. A, B and C. Group A served as control and received normal diet, Group B received piroxicam 0.3 mg /Kg body weight intraperitoneally and Group C 1mg/Kg body weight of zinc intraperitoneally and piroxicam in the same dose as group B. After completion of study [6 weeks] animals were scarified and their livers were removed and after processing paraffin section were made and stained with Haematoxylin and Eosin for histological and histochemical examinations and correlate with serum hepatic enzyme level. Haematoxylin and Eosin stained section of group A indicated the normal histology and morphometry, the group B showed distorted hepatic lobular architecture. Central vein and sinusoids was dilated and congested, kupfer cell prominent and pyknotic cells and mono nuclear infiltration were seen. Group C showed altered histological findings comparable to group A. The Histochemical findings of group B showed depletion in glycogen content marked fibrosis of reticulin fibres and increased deposition of calcium phosphate crystals. In group C effect of zinc improve glycogen content and reticulin fiber deposition and decreased deposition of alkaline phosphatase crystals. The serum enzyme level of Alkaline Phosphatase and Serum Gulutamin Phosphatase significantly increased in group B animals and less significantly increase in group C as compared to group B animals. It was concluded that piroxicam in therapeutic dose, was toxic and produced hepatic injury and zinc along with piroxicam improved the hepatic damage